Evaluation of Culture and Sensitivity Patterns of Urinary Tract Infections in Neonates and Children Presenting with Urinary Symptoms in a Tertiary Care Hospital

Dr. Saroj Kumar Thakur, Mr. Amit Kumar Sah ,Dr. Baidhyanath Thakur, Dr. D.N. Sah, Dr. S.K. Jha

Vol. 19, Issue 1, Jan-Jun 2025

Abstract:

The present cross-sectional study was conducted in the Department of Microbiology at Madhesh Institute of Health Sciences (MIHS), Provincial Hospital Janakpur Dham, from January to December 2023, with the objective of evaluating the culture and sensitivity patterns of urinary tract infections (UTIs) in neonates and children presenting with urinary symptoms. A total of 169 patients aged between 0 and 15 years with suspected complicated UTIs were enrolled. Clean-catch mid-stream urine samples were collected and processed using standard microbiological techniques. Culture media such as Blood agar, MacConkey agar, and CLED agar were employed to isolate pure bacterial colonies, and antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion method on Mueller-Hinton agar, following CLSI 2023 guidelines. Out of 169 samples, 27 yielded positive cultures. Escherichia coli was the predominant pathogen, isolated in 59.25% of the positive cases, followed by Pseudomonas aeruginosa (11.11%), Klebsiella pneumoniae (7.40%), and other organisms including Klebsiella oxytoca, Serratia marcescens, Acinetobacter baumannii, Enterococcus faecalis, Proteus mirabilis, and Citrobacter freundii. The findings highlight E. coli as the most prevalent uropathogen in pediatric UTI cases, with a concerning level of antimicrobial resistance, emphasizing the need for judicious antibiotic use and regular surveillance to guide empirical therapy.

DOI: http://doi.org/10.37648/ijrmst.v19i01.003

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